Quantification of brain-derived extracellular vesicles in plasma as a biomarker to diagnose Parkinson's and related diseases
Posted by Leanne Kodsmann on
Human Plasma from Innovative Research was used in the following study:
Quantification of brain-derived extracellular vesicles in plasma as a biomarker to diagnose Parkinson's and related diseases
Takuma Ohmichi, Masato Mitsuhashi, Harutsugu Tatebe, Takashi Kasai, Omar M. Ali El-Agnaf, Takahiko Tokuda
Parkinsonism & Related Disorders
Volume 61, April 2019, Pages 82-87
"... In brief, forty μL each of standards, controls, and plasma samples were applied to each well of ELISA plates with immobilized monoclonal antibodies against SNAP25, EAAT1, or OMG, and incubated overnight. ... Since our sandwich ELISA used a combination of 2 different target antibodies, such as anti-CD81 and anti-SNAP25, recombinant protein was not applicable as a quantification standard. Thus, we first screened various plasma samples obtained from commercial sources (Innovative Research, Novi, MI ..."
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