*Human Total Factor XII ELISA Kit

Select this product for Molecular Innovations name, Human Coagulation Factor XII Total Antigen ELISA Kit and product number, HFXIIKT-TOT.

Human Total Factor XII ELISA Kit from Innovative Research is intended for the quantitative determination of total Factor XII antigen in human plasma and serum. Strip well format. Reagents for up to 96 tests.

Human Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, antihuman Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor XII present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm.

• Detection Target: Total Factor XII
• Reactivity: Human
• Cross-Reactivity: At recommended dilution no significant cross-reaction occurs with rat, porcine, dog, rabbit or sheep. Please note, pooled normal plasma from mouse resulted in significant background color development
• Range: 0.5 -100 ng/ml
• Sensitivity: 0.098 ng/ml
• Storage Conditions: 2-8°C

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Click Here to Download a Copy of the Kit Manual