Innovative Research offers a large selection of ELISA kits that are ultra-sensitive and can be performed in under 90 minutes. The kits are applicable to a wide range of research areas from coagulation to fibrinolysis. Some of the most frequently asked questions about our ELISA kits have to do with how the signal should look once the test is performed. For more information about standard curves or issues, take a look at the charts below.
Ideal Plate / Assay Turnout
Standard curve in duplicates including
Zero’s (row A and row B), unknowns in
duplicates remaining rows
High Signal Throughout,
Including Standard Curve
Caused By:
• Improper washing
• Skipping a step in the protocol
• Wrong concentration or volume added at a particular step
• Prolonged incubation
No Signal,
Throughout Including Standard Curve
Caused By:
• Improper washing
• Skipping a step in the protocol
• Improper reagent preparation
• Insufficient incubation period
Low Precision,
Improper Standard Curve Fit
Caused By:
• Improper washing
• Improper loading the wells/splashing/ inconsistent volumes
• Insufficient mixing of reagent diltions
• Dilution error
• Contamination of reagents (wash buffer, diluent)
Some of our most popular kits include:
- PAI-1 Antigen Assay (human, mouse, rat, rabbit, dog)
- tPA Assay (Human, mouse, rat)
- uPA Assay (Human, mouse, rat)
- Plasminogen (Human, mouse, monkey)
- Fibrinogen (Human, mouse, rat, rabbit, pig)
To inquire about products from Innovative Research reach out to sales@innov-research.com or call us at (+1)248-896-0145 or toll-free at 888-660-6866.
