Platelet-Primed Interactions of Coagulation and Anticoagulation Pathways in Flow-Dependent Thrombus Formation
Posted by Adam Awdish on
Human Protein C Activated Purified GLA Domainless from Innovative Research was used in the following study:
Sanne L. N. Brouns, Johanna P. van Gefen, Elena Campello, Frauke Swieringa, Luca Spiezia, René van Oerle, Isabella Provenzale, Remco Verdoold, Richard W. Farndale, Kenneth J. Clemetson, Henri M. H. Spronk, Paola E. J. van der Meijden, Rachel Cavill, Marijke J. E. Kuijpers, Elisabetta Castoldi, Paolo Simioni & Johan W. M. Heemskerk
Scientific Reports (Nature Publisher Group)
2020
During the processes of haemostasis and thrombosis, platelet and coagulation pathways are tightly linked, with platelets both responding to thrombin and fibrin and providing a phosphatidylserine-exposing surface on which high levels of thrombin and fibrin are formed. Thrombotic and bleeding disorders are commonly related to a dysregulation of one of these pathways, and in the last decade, it has become clear that the accumulation of a platelet- and fibrin-containing plug or thrombus is instrumental to the onset of both haemostatic and thrombotic events.
An important factor in thrombus development is provided by the local blood flow; For adequate and comprehensive monitoring of the thrombotic process, measurements are to be performed under conditions of flow, preferentially recording both platelet and coagulation activation at the same time.
In this study, researchers looked to identify and characterize principal pathways that regulate the prothrombotic interactions between platelet and coagulation activation in flowing whole blood. A microfluidics technique was developed, allowing for kinetic measurements of adhesion, activation and aggregation of platelets as well as fibrin formation during thrombus build-up. By triggering specific (anti)coagulation pathways, and by time-dependent measurements using multiple fluorescent probes, researchers generated time profiles of all these processes simultaneously. After analyzing blood samples from patients with congenital defects in coagulation factors, scientists confirmed the pathophysiological relevance of alterations in thrombus formation parameters.
Related products available from Innovative Research also include: