Bovine Deoxyribonuclease I RNase/Protease Free

Bovine Deoxyribonuclease I RNase/Protease Free from Innovative Research has been chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter approximately 1 mg/ml containing 50% glycerol and 1 mM calcium chloride. This is a liquid. This product is suitable for use in techniques requiring digestion of DNA in the recovery of intact RNA or where the integrity of structural proteins or enzymes must be maintained. Applications have included nick translation, DNA mapping, isolation of nuclear RNA and protein, RNA polymerase synthesis of RNA probes and RT-PCR. It is also used in tissue culture work to digest DNA from damaged cells thereby reducing viscosity, and removing membrane bound DNA fragments. Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher. As measured here, one Kunitz unit digests 1 Ąg of calf thymus DNA in 10 minutes at 37░C in 50 mM Tris, 1 mM Mg2+, 1 mM Ca2+, pH 7.8. Correlation of digestion units with Kunitz units is different for other DNA and buffer systems. Molecular Biology Grade. More Details: Source: Bovine Pancreas Purification: chromatographically purified Storage Conditions: 2-8░C or -20░C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.