Human Neutrophil Elastase Purified Lyophilized

Human Neutrophil Elastase Purified Lyophilized from Innovative Research is fractionated with NaCl and treated with DNase to release DNA-bound proteins. The elastase is purified by affinity chromatography on Agarose-DAPSA-Elastin Soluble and separated from cathepsins by ion exchange chromatography on carboxymethyl-agarose. The elastase is further purified by affinity chromatography at -15°C on agarose-alanyl-alanyl-alanine. The elastase is free of cathepsin G activity when assayed with Suc-Ala-Ala-Pro-Phe-pNA.Free of cathepsin G, MPO and lysozyme..

Elastase in an enzyme that breaks down elastin, breaks down cytokines and alpha proteinase inhibitor, and cleaves immunoglobulins A and G (IgA/IgG). Elastase is inhibited by alpha-1-antitrypsin (A1AT).

More Details:

• Source: Leucocytes of Purulent Human Sputum
• Purification: Affinity Purified
• Purity: Greater than 95% by SDS-PAGE
• Storage Conditions:Store at 5°C. Stable one year
• Diluents and Stability: 1. 1mg/ml in 0.05 M NaOAc pH 5 containing 0.1 M NaCl. Stable 7 days at 5°C (in ice). 2. 10mg/ml in 50% glycerol 50% 0.02 M NaOAc pH 5. Stable at least 60 days at -20°C with 20 cycles between -20°C and 5°C. 3. May be dissolved at 10mg/ml in 2 mM HoAc and re-lyophilized in smaller quantities.

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Please note - this product replaces our old product number IHNE-SD (same product, new number).