*Human PAI-1 ELISA Kit

Select this product for Molecular Innovations name, Human PAI-1 Activity ELISA Kit and product number, HPAIKT. This human PAI-1 activity assay is for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1) in human plasma. For research use only. Strip well format. Reagents for up to 96 tests. Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate or be detected. Unbound PAI-1 samples are aspirated and an anti-PAI-1 primary antibody is added. Excess primary antibody is then aspirated. The bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample. More Details: Detection Target: PAI-1 Reactivity: Human Range: 0.125-100 U/ml Sensitivity: 0.085 U/ml At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note this product replaces our old product number IHPAIKT (same product, new number).