*Human Total Factor IX ELISA Kit

Select this product for Molecular Innovations name, Human Coagulation Factor IX Total Antigen ELISA Kit and product number, HFIXKT-TOT.

This human coagulation Factor IX antigen assay is intended for the quantitative determination of total Factor IX antigen in human plasma. For research use only. Strip well format. Reagents for up to 96 tests.

Human Factor IX will bind to the affinity purified capture antibody coated on the microtiter plate. Factor IX and IXa will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Factor IX primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor IX present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor IX. Color development is proportional to the concentration of Factor IX in the samples.

More Details:

Detection Target: Factor IX

Reactivity: Human

Range: 0.1-100 ng/ml

Sensitivity: 0.044 ng/ml

Storage Conditions: 4C, the secondary antibody must be stored at -70C.

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Please note this product replaces our old product number IHFIXKT-TOT (same product, new number).

Click Here to Download a Copy of the Kit Manual