*Human Total Factor VIII ELISA Kit

Select this product for Molecular Innovations name, Human Coagulation Factor VIII Total Antigen ELISA Kit and product number, HFVIIIKT-TOT.

This human coagulation Factor VIII antigen assay is intended for the quantitative determination of total Factor VIII antigen in human plasma. For research use only. Strip well format. Reagents for up to 96 tests.

Human Factor VIII will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human Factor VIII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human plasma. Color development is proportional to the concentration of Factor VIII in the samples.

More Details:

Detection Target: Factor VIII

Reactivity: Human

Range: 0.0018?0.91 IU/ml

Sensitivity: 0.00029 IU/ml

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

Please note this product replaces our old product number IHFVIIIKT-TOT (same product, new number).

Click Here to Download a Copy of the Kit Manual