Canine Serum Amyloid A ELISA Kit

Canine Serum Amyloid A ELISA Kit from Innovative Research s a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Canine Serum Amyloid A in biofluid samples. Strip well format. Reagents for up to 96 tests. In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP.) are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Detection Target: Serum Amyloid A Reactivity: Canine Range: 6.25 ng/ml - 200 ng/ml Sensitivity: Storage Conditions: 4C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.