Powered by BiozHuman Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit
IHUEPCAMKTThis Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit from Innovative Research is intended for quantitative detection of human EPCAM in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This human EPCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPCAM has been precoated onto 96-well plates. Standards(NSO, Q24 - K265) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EPCAM amount of sample captured in plate.
- Detection Target: Epithelial Cell Adhesion Molecule (EPCAM)
- Uniprot ID: P16422)
- Reactivity: Human
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 15.6pg/ml-1000pg/ml
- Sensitivity: <10pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.; Immunogen sequence: 369
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