Human Intelectin-1 (Omentin) ELISA Kit

This Human Intelectin-1 (Omentin) ELISA Kit from Innovative Research is intended for quantitative detection of human Omentin/intelectin-1 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.

This human Omentin/intelectin-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Omentin/intelectin-1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: T19-S298) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Omentin/intelectin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Omentin/intelectin-1 amount of sample captured in plate.

• Detection Target: Intelectin-1 (Omentin)
• Uniprot ID: Q8WWA0)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Intelectin-1, also known as omentin or intestinal lactoferrin receptor, is an intelectin encoded in humans by the ITLN1 gene. It is mapped to 1q23.3. Intelectin-1 functions both as a receptor for bacterial arabinogalactans and for lactoferrin. Having conserved ligand binding site residues, both human and mouse intelectin-1 bind the exocyclic vicinal diol of carbohydrate ligands such as galactofuranose.; Immunogen sequence: 369