Mouse Chemokine Ligand CXCL5 Rapid ELISA Kit

This Mouse Chemokine Ligand CXCL5 Rapid ELISA Kit from Innovative Research is intended is a fast ELISA kit, this assay takes less than 1.5 hours to detect Mouse Ena-78/CXCL5 with <10pg/ml sensitivity. format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma (heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Ena-78/CXCL5 tissue specificity: Strip well format. Reagents for up to 96 tests.

This mouse CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL5 has been precoated onto 96-well plates. Standards(E.coli, V45-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL5 amount of sample captured in plate.

• Detection Target: Chemokine Ligand CXCL5
• Uniprot ID: P50228)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. Expression system for standard: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.; Immunogen sequence: 383