Rat Matrix Metalloproteinease-9 (92 kDa Type IV Collagenase) ELISA Kit

This Rat Matrix Metalloproteinease-9 (92 kDa Type IV Collagenase) ELISA Kit from Innovative Research is intended for quantitative detection of rat MMP-9 in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.

This rat MMP-9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-9 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L35-P708) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MMP-9 amount of sample captured in plate.

• Detection Target: Matrix Metalloproteinease-9 (92 kDa Type IV Collagenase)
• Uniprot ID: P50282)
• Reactivity: Rat
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 312pg/ml-20000pg/ml
• Sensitivity: <15pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. ; Immunogen sequence: 369