Mouse Alpha 1 Antitrypsin (A1AT) ELISA Kit

The Mouse Alpha 1 Antitrypsin (A1AT) ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring A1AT in Mouse biological samples. Strip well format. Reagents for up to 96 tests. In this assay the A1AT present in samples reacts with the anti-A1AT antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-A1AT antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1AT . Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1AT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1AT in the test sample. The quantity of A1AT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. More Details: Detection Target: Alpha 1-Antitrypsin Reactivity: Mouse Range: 6.25 ng/ml - 200 ng/ml Sensitivity: 1.492 ng/ml At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note ¢ this product replaces our old product number IRKTAH1147 (same product, new number).