Effects of Different Separation Methods on the Physical and Functional Properties of Extracellular Vesicles
Posted by Adam Awdish on
Pooled Human AB Serum Off The Clot from Innovative Research was used in the following study:
Hyungtaek Jeon, Su-Kyung Kang, Myung-Shin Lee
PLoS ONE
July 7, 2020
Extracellular vesicles (EVs) are nano-sized membrane vesicles (20–500 nm) of endocytic origin that are secreted by most cells under normal physiological conditions, as well as by cells undergoing pathological processes. EVs contain a variety of molecules such as proteins, nucleic acids, and lipids, which are affected by environmental factors and health conditions, making them potential biomarkers for diseases such as cancer.
There are various methods used to isolate EVs from body fluid and cell culture supernatant, which have been compared in prior studies and determined that the component yield and physical properties of isolated EVs depend largely on the isolation method used. Several novel methods have been recently developed which have not yet been compared to the established methods.
The purpose of this study is to compare the physical and functional differences in EVs isolated using a differential centrifugation method, the precipitation-based Invitrogen kit, the ExoLutE kit, and the Exodisc, of which the latter two were recently developed.
Researchers found that Exodisc was the most suitable method for obtaining large quantities of EVs, which might be useful for biomarker investigations, and that the EVs separated using Exodisc exhibited the highest complement activation activity. Results indicated that effective isolation is necessary to study EVs as tools for diagnosing cancer, and these findings may have relevant implications in the field of oncology by providing researchers with data to assist their selection of a suitable isolation method.
Related products available from Innovative Research also include:
Pooled Human AB Serum Plasma Derived