Easy as PAI(-1): Celebrate Pi Day with Cutting-Edge Monoclonal Antibodies from Innovative Research
Posted by Adam Awdish on
In the ever-evolving landscape of molecular biology, precision tools are essential for elucidating complex biological processes. Specifically, when looking at scientific advancement in the field of diagnostic development, the role of specific monoclonal antibodies in understanding and manipulating biological processes cannot be overstated. Innovative Research offers a suite of monoclonal antibodies, including an array of PAI-1 monoclonals, designed to empower researchers in their quest for scientific discovery.
What is PAI-1?
Plasminogen Activator Inhibitor-1 (PAI-1) is a protein that functions as the principal inhibitor of tissue plasminogen activator and urokinase plasminogen activator, two enzymes responsible for the conversion of plasminogen to plasmin (Sillen & Declerck, 2020). Through this inhibitory action, PAI-1 plays a critical role in the regulation of fibrinolysis, the process by which the body breaks down blood clots. Abnormal levels of PAI-1 are associated with increased risk of thrombotic events and have been implicated in various conditions such as cardiovascular disease, obesity, and certain types of cancer (Alessi & Juhan-Vague, 2006, Alessi & Juhan-Vague, 1997).
Mouse Anti-Human PAI-1 Monoclonal Antibody Clone 3C311:
Key Features:
Host and Reactivity: Originating from mouse cells, this monoclonal antibody exhibits high reactivity with human, rat, rabbit, and porcine PAI-1, ensuring versatility across various experimental setups.
Applications: Clone 3C311 is compatible with a range of analytical tools, including ELISA kits and Western blot. It demonstrates robust reactivity with both free and complexed forms of PAI-1, making it invaluable for researchers investigating PAI-1's involvement in disease processes and biological regulation.
Storage: To preserve its integrity and efficacy, the antibody is stored at -70°C and buffered in 0.05M Sodium Phosphate, 0.1M NaCl, 1mM EDTA, pH 6.6.
Potential Applications:
Research Tool: Clone 3C311 serves as a valuable tool for dissecting the intricate role of PAI-1 in fibrinolysis, tumorigenesis, and metabolic syndrome. Understanding these mechanisms can unveil new therapeutic targets (Boren, et al., 2017).
Diagnostic Tool: Given PAI-1's association with various diseases, Clone 3C311 holds promise for developing diagnostic assays to measure PAI-1 levels in patients, offering insights into disease progression or risk.
Therapeutic Exploration: Exploring the modulation of PAI-1 activity through this monoclonal antibody could open novel therapeutic avenues, particularly in conditions where PAI-1 plays a significant role, such as cardiovascular diseases and certain cancers.
Mouse Anti-Human VLDL Receptor Monoclonal Antibody Clone 5F3:
Key Features:
Host and Reactivity: Derived from mouse cells, this monoclonal antibody exhibits high specificity for the Very-low-density Lipoprotein (VLDL) receptor ligand binding domain in humans.
Applications: Clone 5F3 is well-suited for use in ELISA kits and Western blot assays, offering reliable detection under reducing and non-reducing conditions.
Storage: The antibody is stored at -70°C and buffered in 0.05M Sodium Phosphate, 0.1M NaCl, 1mM EDTA, pH 6.6 to maintain stability and efficacy.
Potential Applications:
Metabolic Research: Clone 5F3 facilitates investigations into lipid metabolism and cardiovascular health by enabling precise detection and characterization of VLDL receptor-related molecules.
Disease Modeling: Its efficacy in detecting VLDL receptor ligand binding domain aids in disease modeling and therapeutic intervention studies, providing insights into VLDL receptor biology in various pathologies.
Mouse Anti-Human Vitronectin Monoclonal Antibody Clone 1D144:
Key Features:
Host and Reactivity: Derived from mouse cells, Clone 1D144 exhibits high specificity for human vitronectin, enabling precise investigations into cellular adhesion and extracellular matrix dynamics.
Applications: This monoclonal antibody is compatible with ELISA, Western blot, inhibition, and blocking assays, offering versatility in studying vitronectin-mediated processes.
Storage: To ensure stability and consistency, the antibody is stored at -70°C and buffered in 0.05M Sodium Phosphate, 0.1M NaCl, 1mM EDTA, pH 6.6.
Potential Applications:
Cancer Research: Clone 1D144 facilitates studies on cancer cell migration and invasion by disrupting smooth muscle and endothelial cell adhesion to vitronectin, offering insights into tumor metastasis.
Biomedical Engineering: Its efficacy in blocking vitronectin-mediated adhesion may find applications in designing biomaterials for tissue engineering and regenerative medicine.
Mouse Anti-Human Factor XI Heavy Chain Monoclonal Antibody Clone 9C9:
Key Features:
Host and Reactivity: Derived from mouse cells, Clone 9C9 exhibits high affinity and specificity for human Factor XI, making it an essential tool for investigating coagulation cascades and hemostasis.
Applications: Suitable for ELISA, Western blot, and inhibition assays, Clone 9C9 facilitates precise investigations into Factor XI-mediated processes.
Storage: To maintain integrity and performance, the antibody is stored at -70°C and buffered in 0.05M Sodium Phosphate, 0.1M NaCl, 1mM EDTA, pH 6.6.
Potential Applications:
Hemostasis Research: Clone 9C9 enables researchers to dissect the complexities of Factor XI biology, offering insights into hemostatic regulation and thrombotic disorders.
Clinical Diagnostics: Its efficacy in detecting Factor XI levels may aid in the development of diagnostic assays for monitoring coagulation disorders and assessing thrombotic risk.
Mouse Anti-Human tPA Heavy Chain Monoclonal Antibody Clone 2A153:
Key Features:
Host and Reactivity: Originating from mouse cells, Clone 2A153 exhibits high reactivity with human and pig tissue plasminogen activator (tPA) heavy chain, facilitating investigations into fibrinolysis and thrombosis.
Applications: Compatible with ELISA kits and Western blot assays, Clone 2A153 offers unparalleled sensitivity and specificity for detecting both free and complexed forms of tPA.
Storage: To ensure stability and reliability, the antibody is stored at -70°C and buffered in 0.05M Sodium Phosphate, 0.1M NaCl, 1mM EDTA, pH 6.6.
Potential Applications:
Cardiovascular Research: Clone 2A153 enables researchers to unravel the complexities of fibrinolytic pathways, offering insights into cardiovascular health and disease.
Drug Development: Its efficacy in detecting tPA levels may facilitate drug discovery efforts targeting fibrinolysis modulation for thrombolytic therapy and cardiovascular disease management.
References:
Juhan-Vague, I., & Alessi, M. C. (1997). PAI-1, obesity, insulin resistance and risk of cardiovascular events. Thrombosis and haemostasis, 78(1), 656–660. PMID: 9198234
Alessi, M. C., & Juhan-Vague, I. (2006). PAI-1 and the metabolic syndrome: links, causes, and consequences. Arteriosclerosis, thrombosis, and vascular biology, 26(10), 2200-2207. DOI: 10.1161/01.ATV.0000242905.41404.68
Jake Boren, Grant Shryock, Alexis Fergis, Ann Jeffers, Shuzi Owens, Wenyi Qin, Kathleen B. Koenig, Yoshikazu Tsukasaki, Satoshi Komatsu, Mitsuo Ikebe, Steven Idell, Torry A. Tucker, (2017). Inhibition of Glycogen Synthase Kinase 3β Blocks Mesomesenchymal Transition and Attenuates Streptococcus pneumonia–Mediated Pleural Injury in Mice, The American Journal of Pathology, Volume 187(11), 2461-2472. Retrieved from https://doi.org/10.1016/j.ajpath.2017.07.007.
Sillen, M., & Declerck, P. J. (2020). Targeting PAI-1 in Cardiovascular Disease: Structural Insights Into PAI-1 Functionality and Inhibition. Frontiers in Cardiovascular Medicine, 7, Figure 4. https://doi.org/10.3389/fcvm.2020.622473