Assay and ELISA Kits

Select this product for Molecular Innovations name, Mouse Fibrinogen Antigen Sandwich ELISA Kit and product number, MFBGNKT.

This mouse fibrinogen antigen assay is intended for the quantitative determination of total fibrinogen antigen in mouse plasma and serum. For research use only. Strip well format. Reagents for up to 96 tests.

Mouse fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse fibrinogen. Color development is proportional to the concentration of fibrinogen in the samples.

More Details:

Detection Target: Fibrinogen

Reactivity: Mouse

Range: 3.125-800 ng/ml

Sensitivity: 0.289 ng/ml

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

Please note: this product replaces our old product number IMFBGNKT (same product, new number).

Click Here to Download a Copy of the Kit Manual

Select this product for Molecular Innovations name, Human Fibrinogen Antigen Sandwich ELISA Kit and product number, HFIBKT.

This human fibrinogen antigen assay is intended for the quantitative determination of total fibrinogen antigen in human plasma and serum. For research use only. Strip well format. Reagents for up to 96 tests.

Human fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-human fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with streptavidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human fibrinogen. Color development is proportional to the concentration of fibrinogen in the samples.

More Details:

Detection Target: Fibrinogen

Reactivity: Human

Range: 3.125-800 ng/ml

Sensitivity: 0.41 ng/ml

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

Please note: this product replaces our old product number IHFBGNKT (same product, new number).

Click Here to Download a Copy of the Kit Manual

Select this product for Molecular Innovations name, Human IgG Antigen Sandwich ELISA Kit and product number, HUIGGKT. This human immunoglobulin G antigen assay is intended for the quantitative determination of total human IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgG subclasses. For research use only. Strip well format. Reagents for up to 96 tests. Human IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgG antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human IgG. Color development is proportional to the concentration of IgG in the samples.

More Details:

Detection Target: IgG

Reactivity: Human

Range: 0.2-200 ng/ml

Sensitivity: 0.088 ng/mL

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

Please note this product replaces our old product number IHUIGGKT (same product, new number).

Click here to watch an informative tutorial that details instructions for running this kit.

Select this product for Molecular Innovations name, SARS-CoV-2 IgG Seroconversion Sandwich ELISA Kit and product number, SARSCOV2GKT.

This SARS-CoV-2 IgG Seroconversion Sandwich ELISA Kit from Innovative Research is intended for the qualitative determination of SARS-CoV-2 specific antibodies of IgG class in plasma or serum samples. Strip well format. Reagents for up to 96 tests. For research only.

This assay has been specifically developed using the SARS-CoV-2 receptor binding domain (RBD) of the spike protein. This delivers reliable and consistent results with a high degree of reproducibility.

This assay can be performed in 70 minutes, and has an overall sensitivity of 97-100% and specificity of 100%.

Principle of the Assay:

SARS-CoV-2 specific antibodies will bind to the purified recombinant HEK cell derived receptor-binding domain (RBD) of the SARS-CoV-2 spike protein coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgG secondary antibody binds to the captured protein. Excess secondary antibody is washed away and TMB substrate is used for color development at 450 nm. Samples that exceed a determined cut-off value are designated positive by this assay.

More Details:

• Detection Target: SARS-CoV-2 specific antibodies of isotype IgG
• Dilution of Samples: A 1:51 dilution of serum samples is suggested for best results.
• Expected Values: Significant seroconversion as demonstrated by antibodies specific to both the full-length SARS-CoV-2 spike protein and the RBD region has been detected as early as two days post symptom onset.

• Sensitivity: 97% for samples obtained more than 14 days after symptom onset (n=37), 100% for samples obtained more than 21 days after symptom onset (n=32).

• Specificity: 100% (n=89)

• Percent Agreement: Positive 100%; Negative 91%

• Storage: Store all kit components at 4°C upon arrival. Return any unused microplate strips to the plate pouch with desiccant. Reconstituted controls may be stored at -80°C for later use. Store all other unused kit components at 4°C. This kit should not be used beyond the expiration date.

Negative results do not preclude acute SARS-CoV-2 infection. If acute infection is suspected, direct testing for SARS-CoV-2 is necessary. Results from antibody testing should not be used to diagnose or exclude acute SARS-CoV-2 infection. Positive results may be due to past or present infection with non-SARS-CoV-2 coronavirus strains, such as KHU1, NL63, OC43, or 229E. This serological assay can be used for applications like characterizing immune responses to viral infection by determining the presence of viral antigen-specific antibodies in infected and recovered patient sera. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

Select this product for Molecular Innovations name, Human Coagulation Factor XII Total Antigen Sandwich ELISA Kit and product number, HFXIIKT-TOT.

Human Total Factor XII Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of total Factor XII antigen in human plasma and serum. Strip well format. Reagents for up to 96 tests.

Human Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, antihuman Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor XII present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm.

• Detection Target: Total Factor XII
• Reactivity: Human
• Cross-Reactivity: At recommended dilution no significant cross-reaction occurs with rat, porcine, dog, rabbit or sheep. Please note, pooled normal plasma from mouse resulted in significant background color development
• Range: 0.5 -100 ng/ml
• Sensitivity: 0.098 ng/ml
• Storage Conditions: 2-8°C

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

Click Here to Download a Copy of the Kit Manual

Select this product for Molecular Innovations name, Human PAI-1 Total Antigen Sandwich ELISA Kit and product number, HPAIKT-TOT. This human plasminogen activator inhibitor type 1 (PAI-1) total antigen assay is intended for the quantitative determination of total PAI-1 in human plasma in citrate, EDTA or heparin, serum, cell culture media, tissue lysates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. Human PAI-1 will bind to the monoclonal capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will bind to the plate. After appropriate washing steps, polyclonal anti-human PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human PAI-1. Color development is proportional to the concentration of PAI-1 in the samples.

More Details:

Detection Target: PAI-1

Reactivity: Human

Range: 0.25-100 ng/ml

Sensitivity: 0.122ng/ml

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

Please note: this product replaces our old product number IHPAIKT-TOT (same product, new number).

The Human Complement Component 1q (C1q) Sandwich ELISA Kit employs a quantitative enzyme immunoassay technique that measuresComplement Component 1q (C1q) in Human samples. Strip well format. Reagents for up to 96 tests.

It is designed for detection of complement C1q in human plasma, serum, milk, urine, saliva, CSF, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human complement C1q in less than 4 hours. A polyclonal antibody specific for human complement C1q has been pre-coated onto a 96-well microplate with removable strips. Complement C1q in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human complement C1q, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

More Details:

• Detection Target: Complement Component 1q (C1q)
• Reactivity: Human
• Range: 90 pg/ml
• Sensitivity: 90 pg/ml

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

Please note – this product replaces our old product number IRAPKT4011 (same product, new number).

Select this product for Molecular Innovations name, Human Complement C3 Antigen Sandwich ELISA Kit and product number, HC3KT.

Human Total Complement Component 3 (C3) Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of total C3 antigen in human plasma, serum, urine, milk, saliva and cell culture samples. Strip well format. Reagents for up to 96 tests.

Human C3 will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human C3 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm.

• Detection Target: Total Complement Component 3 (C3)
• Reactivity: Human
• Cross-Reactivity: At recommended dilution no significant cross-reaction occurs with mouse, rat, Porcine, sheep, or horse.
• Range: 0.02-10 ng/ml
• Sensitivity: 0.011 ng/mL
• Storage Conditions: 2-8°C

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.