*E.coli DH5-alpha Competent Cells

Select this product for Molecular Innovations name, DH5-alpha Competent Cells and product number, DH5A

E.coli DH5-alpha Competent Cells from Innovative Research are an all purpose reagent for general cloning, sub-cloning and plasmid isolation. For a transformation efficiency of at least 1x106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate.

More Details:

• Strain: E.coli
• Format: Frozen Liquid
• Storage: -70C
  
• Volume: 10 x 0.1 ml
• Aliquot: 1 x 1.0 ml
  

Additional Information:
For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 10 single use 0.1 ml aliquots.

Select this product for Molecular Innovations name, DH5-alpha Competent Cells and product number, DH5A