Select this product for Molecular Innovations name, DH5-alpha Competent Cells and product number, DH5A

E.coli DH5-alpha Competent Cells from Innovative Research are an all purpose reagent for general cloning, sub-cloning and plasmid isolation. For a transformation efficiency of at least 1x106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate.

More Details:

• Strain: E.coli
• Format: Frozen Liquid
• Storage: -70C
  
• Volume: 10 x 0.1 ml
• Aliquot: 1 x 1.0 ml
  

Additional Information:
For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 10 single use 0.1 ml aliquots.

Select this product for Molecular Innovations name, DH5-alpha Competent Cells and product number, DH5A

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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