The Horse Serum Amyloid A (SAA) ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAA in Horse biological fluids. Strip well format. Reagents for up to 96 tests. In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-SAA, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. More Details: Detection Target: Serum Amyloid A Reactivity: Horse Range: 2.25 ng/ml - 72 ng/ml Sensitivity: 0.962 ng/ml At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note ¢ this product replaces our old product number IRAPKT3103 (same product, new number).

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