-
⚡️ Last Chance Sale ⚡️ Last Chance Sale ⚡️ Last Chance Sale ⚡️ Last Chance Sale⚡️ Last Chance Sale ⚡️ Last Chance Sale ⚡️ Last Chance Sale ⚡️ Last Chance Sale⚡️ Last Chance Sale ⚡️ Last Chance Sale ⚡️ Last Chance Sale ⚡️ Last Chance Sale
Human Tryptase ELISA Kit
Human Tryptase ELISA Kit
SKU:IHUTPSKT
Couldn't load pickup availability
Questions about this product or need to customize? Contact us
In-depth information
Certificate of Analysis
Contact customer support to get your certificate of analysis
This Human Tryptase ELISA Kit from Innovative Research is intended for quantitative detection of human Tryptase in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This human Tryptase ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Tryptase has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: M1-V275) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tryptase is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Tryptase amount of sample captured in plate.
- Detection Target: Tryptase
- Uniprot ID: Q15661, P20231)
- Reactivity: Human
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 156pg/ml-10000pg/ml
- Sensitivity: <15pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a polyclonal antibody from goat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.; Immunogen sequence: 369
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
Please be aware the image pictured is for illustrative purposes only, and your product packaging and appearance may vary.
Publications
and citations
The latest in science and research
-
Product References
Discover the Innovative Research Product Catalog: Watch Now to Explore Our Offerings
By Adam Awdish On Dec 02, 2024Looking to optimize your research tools and set your lab up for success? Our recent YouTube video, hosted by Maria...
-
Product References
Breakthrough ELISA & CLIA Kits from Innovative Research
By Adam Awdish On Aug 16, 2024At Innovative Research, we're committed to providing cutting-edge tools that empower your scientific discoveries. Our premium selection of human immunoassay...
-
Product References
In Stock Alert: Human and Animal-sourced tPA
By Wendy Wise On Jun 05, 2024Understanding Human Tissue Plasminogen Activator (tPA) tPA is an essential enzyme in the body that helps break down blood clots...