Porcine IgG ELISA Kit

Porcine IgG ELISA Kit from Innovative Research is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Porcine IgG in biological samples. Minimum reactivity to Human IgG. Strip well format. Reagents for up to 96 tests. In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Detection Target: IgG

Reactivity: Porcine

Range: 15.625 ng/ml - 1000 ng/ml

Sensitivity: 7.080 ng/ml

Storage Conditions: 4C

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