*Mouse Active tPA ELISA Kit

Select this product for Molecular Innovations name, Mouse tPA Activity ELISA Kit and product number, MTPAKT. This mouse tPA activity assay is intended for the quantitative determination of active tissue plasminogen activator in mouse plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. Functionally active mouse tPA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. A standard calibration curve is prepared using dilutions of tPA along with the samples to be measured. After appropriate washing steps, monoclonal anti-mouse tPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample. More Details: Detection Target: tPA Reactivity: Mouse Range: 0.05-50 ng/ml Sensitivity: 0.02 ng/ml At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note ŌĆō this product replaces our old product number IMTPAKT (same product, new number).