On-substrate Enzymatic Reaction to Determine Acetylcholinesterase Activity in Whole Blood by Paper Spray Mass Spectrometry
Posted by Leanne Kodsmann on
Single Donor Human Whole Blood from Innovative Research was used in the following study:
Daniel O. Carmany, Phillip M. Mach, Gabrielle M. Rizzo, Elizabeth S. Dhummakupt, Ethan M. McBride, Jennifer W. Sekowski, Bernard Benton, Paul S. Demond, Michael W. Busch, Trevor Glaros
Journal of The American Society for Mass Spectrometry
December 2018 (Volume 29, Issue 12, pp 2436-2442)
"... Hydrophobic paper surfaces were prepared using vaporized trichloro(3,3,3-trifluoropropyl)silane. In both approaches, mixtures of diluted human whole blood with and without VX were mixed with a non-endogenous AChE specific substrate, 1,1-dimethyl-4-acetylthiomethylpiperidinium (MATP+). Formation of the cleaved MATP+ product was monitored over time and compared to MATP+ to determine relative AChE activity...Human blood was purchased from Innovative Research (Novi, MI, USA) ..."
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