Nucleic Acid Sample Preparation Using Magnetic Ionic Liquids as Cell Lysis and DNA Extraction Solvents
Posted by Adam Awdish on
Pooled Human Plasma Apheresis Derived from Innovative Research was used in the following study:
Miranda N. Emaus
Iowa State University Graduate Theses and Dissertations
2021
When preparing DNA samples from cells with disrupted membranes, the first step on sample preparation is generally cell lysis. DNA is then extracted after cell lysis and then analysis of the nucleic acid is performed. However, current sample preparation methods are time consuming, multi-step processes.
Magnetic ionic liquids (MILs) are salts in a molten form which are susceptible to magnets and are a subclass of ionic liquids (ILs). Like ILs, MILs express other physical properties like negligible vapor pressure and the ability to tune their viscosity.
This study investigated the research potential of MILs in DNA sample preparation. The process included enriching DNA with hexafluoroacetylactonate-based MILs. A magnetic rod was then used to isolate the MIL-enriched DNA samples for analysis.
Related products available from Innovative Research also include:
Pooled Human Serum Off The Clot