A Novel Method of High-Purity Extracellular Vesicle Enrichment from Microliter-scale Human Serum for Proteomic Analysis
Posted by Adam Awdish on
Pooled Human Serum Off The Clot from Innovative Research was used in the following study:
Xiaohui Ji, Sisi Huang, Jie Zhang, Terri F. Bruce, Zhijing Tan, Donglin Wang, Jianhui Zhu, R. Kenneth Marcus, and David M. Lubman
Electrophoresis
November 9, 2020
Extracellular vesicles (EVs) are small, membrane-enclosed vesicles released by cells into extracellular spaces. EVs contain DNA, RNA, and proteins derived from their parent cell, and this combined with their size and ability to easily penetrate biological barriers makes them a unique and important method of communication between cells. Consequently, EVs could potentially prove to be reliable biomarker for early detection of diseases and the delivery of therapeutic reagents to diseased cells.
One topic being studied in relation to EV therapeutics is the characterization of EV cargo, or profiling the specific protein content of an EV for use in biomarker discovery. For protein characterization to happen, the EVs must be separated from the biological fluids they are obtained from, which proves difficult due to their size and properties. Many common methods for EV separation fall short due to lacking ability to fully purify EVs, the need for large sample sizes for purification, and due to being timely processes.
Researchers in this study investigated the possibility of performing a chemically selective type of chromatography on a high-performance liquid chromatography (HPLC) platform. They used a novel polyester capillary channel polymer (PET C-CP) fiber phase/hydrophobic interaction chromatography (HIC) method to separate EVs from less than 100uL of serum with minimal contamination. This novel method yields results in roughly 15 minutes with consistently pure results.
Related products available from Innovative Research also include:
Single Donor Human Platelets ACD